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This paper presents an adaptive focus stacking method for large depth-of-field (DOF) 3D microscopic structured-light imaging systems. Conventional focus stacking methods typically capture images under a series of pre-defined focus settings without considering the attributes of the measured object. Therefore, it is inefficient since some of the focus settings might be redundant. To address this problem, we first employ the focal sweep technique to reconstruct an initial rough 3D shape of the measured objects. Then, we leverage the initial 3D data to determine effective focus settings that focus the camera on the valid areas of the measured objects. Finally, we reconstruct a high-quality 3D point cloud using fringe images obtained from these effective focus settings by focus stacking. Experimental results demonstrate the success of the proposed method.

 

This Letter presents a novel, to the best of our knowledge, method to calibrate multi-focus microscopic structured-light three-dimensional (3D) imaging systems with an electrically adjustable camera focal length. We first leverage the conventional method to calibrate the system with a reference focal lengthf₀. Then we calibrate the system with other discrete focal lengthsf_iby determining virtual features on a reconstructed white plane usingf₀. Finally, we fit the polynomial function model using the discrete calibration results forf_i. Experimental results demonstrate that our proposed method can calibrate the system consistently and accurately.

 

This paper presents a calibration method for a microscopic structured light system with an extended depth of field (DOF). We first employed the focal sweep technique to achieve large enough depth measurement range, and then developed a computational framework to alleviate the impact of phase errors caused by the standard off-the-shelf calibration target (black circles with a white background). Specifically, we developed a polynomial interpolation algorithm to correct phase errors near the black circles to obtain more accurate phase maps for projector feature points determination. Experimental results indicate that the proposed method can achieve a measurement accuracy of approximately 1.0 μ m for a measurement volume of approximately 2,500 μ m (W) × 2,000 μ m (H) × 500 μ m (D). 

Bile duct cancer is the second most common primary liver cancer, with most diagnoses occurring in the advanced stages. This leads to a poor survival rate, which means a technique capable of reliably detecting pre-cancer in the bile duct is urgently required. Unfortunately, radiological imaging lacks adequate accuracy for distinguishing dysplastic and benign biliary ducts, while endoscopic techniques, which can directly assess the bile duct lining, often suffer from insufficient sampling. Here, we report an endoscopic optical light scattering technique for clinical evaluation of the malignant potential of the bile duct. This technique employs an ultraminiature spatial gating fiber optic probe compatible with cholangioscopes and endoscopic retrograde cholangiopancreatography (ERCP) catheters. The probe allowed us to investigate the internal cellular composition of the bile duct epithelium with light scattering spectroscopy (LSS) and phenotypic properties of the underlying connective tissue with diffuse reflectance spectroscopy (DRS). In a pilot in vivo double-blind prospective study involving 29 patients undergoing routine ERCP procedures, the technique detected malignant transformation with 97% accuracy, showing that biliary duct pre-cancer can be reliably identified in vivo non-invasively.